RPL19 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: RPL19 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA020160LA01HU
• Hersteller Artikelnummer: CSB-PA020160LA01HU
• Alternativnummer: CSB-PA020160LA01HU-100UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: 60S ribosomal protein L19 antibody, DKFZp779D216 antibody, FLJ27452 antibody, HGNC:10312 antibody, L19 antibody, MGC71997 antibody, Ribosomal Protein L19 antibody, Ribosomal protein L19, cytosolic, N terminus truncated antibody, RL19_HUMAN antibody, rpl19 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P84098
• Puffer: Preservative: 0.03% Proclin 300<br /> Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: RPL19

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000,IHC:1:20-1:200,IF:1:20-1:200

Bilder

Imm

Immunofluorescence staining of HepG2 cell with CSB-PA020160LA01HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA020160LA01HU diluted at 1:50 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-PA020160LA01HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), MCF7 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), HEK293 whole cell lysate(20µg), HepG2 whole cell lysate(20µg)
All lanes: RPL19 antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 23,28 kDa
Observed band size: 28 kDa
Exposure time: 180s