TGFBR1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA023451LA01HU
Artikelname: TGFBR1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA023451LA01HU
Hersteller Artikelnummer: CSB-PA023451LA01HU
Alternativnummer: CSB-PA023451LA01HU-100UL, CSB-PA023451LA01HU-50UL
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: AAT 5 antibody, AAT5 antibody, Activin A receptor type II like kinase 53kDa antibody, Activin A receptor type II like kinase antibody, Activin A receptor type II like kinase, 53kD antibody, Activin A receptor type II like protein kinase of 53kD antibody, activin A receptor type II-like kinase, 53kDa antibody, activin A receptor type II-like protein kinase of 53kD antibody, Activin receptor like kinase 5 antibody, Activin receptor-like kinase 5 antibody, ACVRLK 4 antibody, ACVRLK4 antibody, ALK 5 antibody, ALK-5 antibody, ALK5 antibody, LDS1A antibody, LDS2A antibody, MSSE antibody, Serine/threonine protein kinase receptor R4 antibody, Serine/threonine-protein kinase receptor R4 antibody, SKR 4 antibody, SKR4 antibody, TbetaR I antibody, TbetaR-I antibody, TGF beta receptor type 1 antibody, TGF beta receptor type I antibody, TGF beta type I receptor antibody, TGF-beta receptor type I antibody, TGF-beta receptor type-1 antibody, TGF-beta type I receptor antibody, TGFBR 1 antibody, TGFBR1 antibody, TGFBR1 protein antibody, TGFR 1 antibody, TGFR-1 antibody, TGFR1 antibody, TGFR1_HUMAN antibody, Transforming growth factor beta receptor 1 antibody, Transforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD) antibody, Transforming growth factor beta receptor I antibody, transforming growth factor, beta receptor 1 antibody, transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD) antibody, Transforming growth factor-beta receptor type I antibody
Klonalität: Polyclonal
UniProt: P36897
Puffer: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: TGFBR1
Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:100-1:300, IF:1:10-1:100
Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y, PC-3 whole cell lysate
All lanes: TGFBR1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56, 57, 48kDa
Observed band size: 58, 40kDa
IHC image of CSB-PA023451LA01HU diluted at 1:1200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cell with CSB-PA023451LA01HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).