TIMM23 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TIMM23 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA023553ESR2HU
• Hersteller Artikelnummer: CSB-PA023553ESR2HU
• Alternativnummer: CSB-PA023553ESR2HU-100UL, CSB-PA023553ESR2HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: TIMM23 antibody, TIM23 antibody, Mitochondrial import inner membrane translocase subunit Tim23 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O14925
• Puffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: TIMM23

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000,IF:1:20-1:200

Bilder

Immunofluorescence staining of A549 cell with CSB-PA023553ESR2HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: JK whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), 293 whole cell lysate(20µg), A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), Mouse Heart tissue lysate(20µg)
All lanes: TIMM23 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 300s