VASN Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: VASN Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA025796OA01HU
• Hersteller Artikelnummer: CSB-PA025796OA01HU
• Alternativnummer: CSB-PA025796OA01HU-100UL, CSB-PA025796OA01HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: 2610528G05Rik antibody, PRO1282 antibody, PRO357 antibody, Protein slit-like 2 antibody, Slit like 2 antibody, Slitl2 antibody, UNQ314 antibody, Vasn antibody, VASN_HUMAN antibody, Vasorin antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q6EMK4
• Puffer: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: VASN

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:100-1:300, IF:1:20-1:100

Bilder

Immunofluorescence staining of MCF-7 cell with CSB-PA025796OA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA025796OA01HU diluted at 1:100 and staining in paraffin-embedded human Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: SY5Y whole cell lysate(30µg), K562 whole cell lysate(30µg), HL60 whole cell lysate(30µg)
All lanes: VASN antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 120s

Immunofluorescence staining of Hela cell with CSB-PA025796OA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).