YWHAQ Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: YWHAQ Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA026290ESR1HU
• Hersteller Artikelnummer: CSB-PA026290ESR1HU
• Alternativnummer: CSB-PA026290ESR1HU-100UL, CSB-PA026290ESR1HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: 14-3-3 protein T cell antibody, 14-3-3 protein T-cell antibody, 14-3-3 protein tau antibody, 14-3-3 protein theta antibody, 1433T_HUMAN antibody, IC5 antibody, Protein HS1 antibody, Protein tau antibody, YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody, Ywhaq antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P27348
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: YWHAQ

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:500, IF:1:50-1:200

Bilder

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA026290ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA026290ESR1HU at dilution of 1:100

Immunofluorescence staining of Hela cells with CSB-PA026290ESR1HU at 1:73, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA026290ESR1HU diluted at 1:221 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot
All lanes: 14-3-3 protein theta antibody at 4ug/ml
Lane 1: A549 whole cell lysate
Lane 2: Mouse lung tissue
Lane 3: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa