BCAP31 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA03425A0RB
- Bilder (5)
| Artikelname: | BCAP31 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA03425A0RB |
| Hersteller Artikelnummer: | CSB-PA03425A0Rb |
| Alternativnummer: | CSB-PA03425A0RB-100UG, CSB-PA03425A0RB-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Konjugation: | Unconjugated |
| Alternative Synonym: | 6C6 AG antibody, 6C6 AG tumor associated antigen antibody, 6C6-AG tumor-associated antigen antibody, 6C6AG antibody, 6C6AG tumor associated antigen antibody, Accessory protein BAP 31 antibody, Accessory protein BAP31 antibody, B cell receptor associated protein 31 antibody, B-cell receptor-associated protein 31 antibody, BA31 antibody, BAP 31 antibody, Bap31 antibody, BAP31_HUMAN antibody, BCAP 31 antibody, BCAP31 antibody, BCR associated protein Bap 31 antibody, BCR associated protein Bap31 antibody, BCR-associated protein 31 antibody, CDM antibody, CDM protein antibody, DXS1357E antibody, MS950 antibody, p28 antibody, p28 Bap31 antibody, Protein CDM antibody, RP23-329M9.5 antibody |
| Klonalität: | Polyclonal |
| UniProt: | P51572 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | BCAP31 |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500, IP:1:200-1:2000 |
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Immunofluorescence staining of Hela cells with CSB-PA03425A0Rb at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating BCAP31 in Mouse liver tissue Lane 1: Rabbit control IgG (1µg) instead of CSB-PA03425A0Rb in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA03425A0Rb (6µg) + Mouse liver tissue (500µg) Lane 3: Mouse liver tissue (10µg) |
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Western Blot Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat lung tissue, Rat brain tissue, Mouse lung tissue, Mouse brain tissue All lanes: BCAP31 antibody at 4.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28, 35 kDa Observed band size: 28 kDa |
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