BMP2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA09419A0RB
Artikelname: BMP2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA09419A0RB
Hersteller Artikelnummer: CSB-PA09419A0Rb
Alternativnummer: CSB-PA09419A0RB-100UG, CSB-PA09419A0RB-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: BDA2 antibody, BMP-2 antibody, BMP-2A antibody, Bmp2 antibody, BMP2_HUMAN antibody, BMP2A antibody, Bone morphogenetic protein 2 antibody, Bone morphogenetic protein 2A antibody
Klonalität: Polyclonal
UniProt: P12643
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: BMP2
Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA09419A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA09419A0Rb at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA09419A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image of CSB-PA09419A0Rb diluted at 1:750 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate
All lanes: BMP2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa