Immunofluorescent analysis of Hela cells using CSB-PA12719A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry analysis of human glioma using CSB-PA12719A0Rb at dilution of 1:100
Immunocytochemistry analysis of human pancreatic tissue using CSB-PA12719A0Rb at dilution of 1:100
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Mouse liver tissue All lanes: CBS antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 62 kDa Observed band size: 61 kDa
Immunoprecipitating CBS in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA12719A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CSB-PA12719A0Rb (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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