SMAD2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA15557A0RB
- Bilder (6)
| Artikelname: | SMAD2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA15557A0RB |
| Hersteller Artikelnummer: | CSB-PA15557A0Rb |
| Alternativnummer: | CSB-PA15557A0RB-100UG, CSB-PA15557A0RB-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Drosophila, homolog of, MADR2 antibody, hMAD-2 antibody, HsMAD2 antibody, JV18 antibody, JV18-1 antibody, JV181 antibody, MAD antibody, MAD homolog 2 antibody, MAD Related Protein 2 antibody, Mad-related protein 2 antibody, MADH2 antibody, MADR2 antibody, MGC22139 antibody, MGC34440 antibody, Mother against DPP homolog 2 antibody, Mothers against decapentaplegic homolog 2 antibody, Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody, Mothers against DPP homolog 2 antibody, OTTHUMP00000163489 antibody, Sma and Mad related protein 2 antibody, Sma- and Mad-related protein 2 MAD antibody, SMAD 2 antibody, SMAD family member 2 antibody, SMAD, mothers against DPP homolog 2 antibody, SMAD2 antibody, SMAD2_HUMAN antibody |
| Klonalität: | Polyclonal |
| UniProt: | Q15796 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | SMAD2 |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:50-1:500 |
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Immunofluorescent analysis of HepG2 cells using CSB-PA15557A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA15557A0Rb at dilution of 1:100 |
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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue All lanes: SMAD2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 49 kDa Observed band size: 53 kDa |
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Immunofluorescence staining of Hela cells with CSB-PA15557A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA15557A0Rb diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: SMAD2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 49 kDa Observed band size: 53 kDa |
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