HDAC1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA17869A0RB
Artikelname: HDAC1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA17869A0RB
Hersteller Artikelnummer: CSB-PA17869A0Rb
Alternativnummer: CSB-PA17869A0RB-100UG, CSB-PA17869A0RB-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, ELISA, IF, IHC
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: DKFZp686H12203 antibody, GON 10 antibody, HD1 antibody, HDAC 1 antibody, HDAC1 antibody, HDAC1_HUMAN antibody, Histone deacetylase 1 antibody, Reduced potassium dependency yeast homolog like 1 antibody, RPD3 antibody, RPD3L1 antibody
Klonalität: Polyclonal
UniProt: Q13547
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: HDAC1
Application Verdünnung: Recommended dilution: IHC:1:20-1:200, IF:1:100-1:500
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA17869A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA17869A0Rb at dilution of 1:100
Immunofluorescence staining of Hela cells with CSB-PA17869A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP17869HU) against the P21 promoter.
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA17869A0Rb at dilution of 1: 100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA17869A0Rb at dilution of 1: 100