Immunofluorescence staining of HepG2 cells with CSB-PA618775LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: FOXE3 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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