Immunofluorescent analysis of HepG2 cells using CSB-PA623101LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA623101LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA623101LA01HU at dilution of 1:100
Immunoprecipitating IMMT in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA623101LA01HU in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA623101LA01HU (8ug) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (10ug)
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate All lanes: IMMT antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84, 83, 81 kDa Observed band size: 84 kDa
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