CRHR2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CRHR2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA623797LA01HU
• Hersteller Artikelnummer: CSB-PA623797LA01HU
• Alternativnummer: CSB-PA623797LA01HU-100UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: Corticotropin releasing hormone receptor 2 antibody, Corticotropin-releasing factor receptor 2 antibody, Corticotropin-releasing hormone receptor 2 antibody, CRF 2 antibody, CRF 2R antibody, CRF R2 antibody, CRF RB antibody, CRF-R-2 antibody, CRF-R2 antibody, CRF2 antibody, CRF2 receptor beta isoform antibody, CRF2R antibody, CRFR 2 antibody, CRFR-2 antibody, CRFR2 antibody, CRFR2_HUMAN antibody, CRH 2R antibody, CRH R2 antibody, CRH-R-2 antibody, CRH-R2 antibody, CRH2R antibody, CRHR 2 antibody, Crhr2 antibody, HM CRF antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q13324
• Puffer: Preservative: 0.02% sodium azide<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: CRHR2

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Bilder

Immunofluorescence staining of Hela cell with CSB-PA623797LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with CSB-PA623797LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA623797LA01HU diluted at 1:150 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: Mouse Brain tissue lysate(20µg),Mouse skeletal muscle tissue lysate(20µg),Rat skeletal muscle tissue lysate(20µg),293 whole cell lysate(20µg), A549 whole cell lysate(20µg)
All lanes: CRHR2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 48,51,46,45,43 kDa
Observed band size: 55 kDa
Exposure time: 120s