VPS26B Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: VPS26B Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA676824LA01HU
• Hersteller Artikelnummer: CSB-PA676824LA01HU
• Alternativnummer: CSB-PA676824LA01HU-100UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Pep8b antibody, Vacuolar protein sorting 26 homolog B (S. pombe) antibody, Vacuolar protein sorting 26 homolog B (yeast) antibody, Vacuolar protein sorting-associated protein 26B antibody, Vesicle protein sorting 26B antibody, VP26B_HUMAN antibody, VPS26B antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q4G0F5
• Puffer: Preservative: 0.02% sodium azide Constituents: PBS containing 50% glycerol pH 7.3
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: VPS26B

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200

Bilder

Immunofluorescence staining of Hela cell with CSB-PA676824LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA676824LA01HU diluted at 1:50 and staining in paraffin-embedded mouse brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

Western Blot
Positive WB detected in: K562 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MCF7 whole cell lysate, A431 whole cell lysate
All lanes: VPS26B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa