PLEKHS1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA716341LA01HU
Artikelname: PLEKHS1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA716341LA01HU
Hersteller Artikelnummer: CSB-PA716341LA01HU
Alternativnummer: CSB-PA716341LA01HU-100UG, CSB-PA716341LA01HU-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: PLEKHS1 antibody, C10orf81 antibody, Pleckstrin homology domain-containing family S member 1 antibody, PH domain-containing family S member 1 antibody, Epididymis luminal protein 185 antibody, hEL185 antibody
Klonalität: Polyclonal
UniProt: Q5SXH7
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: PLEKHS1
Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
Immunohistoch
Western Blot
Positive WB detected in: A549 whole cell lysate,Hela whole cell lysate,MCF7 whole cell lysate,SY5Y whole cell lysate
All lanes: PLEKHS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
IHCimageofCSB-PA716341LA01HUdilutedat1:66andstaininginparaffin-embeddedhumansmallintestinetissueperformedonaLeicaBondTMsystem.Afterdewaxingandhydration,antigenretrievalwasmediatedbyhighpressureinacitratebuffer(pH6.0).Sectionwasblockedwith10%normalgoatserum30minatRT.Thenprimaryantibody(1%BSA)wasincubatedat4Covernight.TheprimaryisdetectedbyaGoatanti-rabbitpolymerIgGlabeledbyHRPandvisualizedusing0.05%DAB.
IHCimageofCSB-PA716341LA01HUdilutedat1:66andstaininginparaffin-embeddedhumanendometrialcancerperformedonaLeicaBondTMsystem.Afterdewaxingandhydration,antigenretrievalwasmediatedbyhighpressureinacitratebuffer(pH6.0).Sectionwasblockedwith10%normalgoatserum30minatRT.Thenprimaryantibody(1%BSA)wasincubatedat4Covernight.TheprimaryisdetectedbyaGoatanti-rabbitpolymerIgGlabeledbyHRPandvisualizedusing0.05%DAB.