NHLRC1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NHLRC1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA740936ESR2HU
• Hersteller Artikelnummer: CSB-PA740936ESR2HU
• Alternativnummer: CSB-PA740936ESR2HU-100UL, CSB-PA740936ESR2HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Rat
• Konjugation: Unconjugated
• Alternative Synonym: bA204B7.2 antibody, E3 ubiquitin-protein ligase NHLRC1 antibody, EPM2A antibody, EPM2B antibody, EPM2B gene antibody, Malin antibody, MGC119262 antibody, MGC119264 antibody, MGC119265 antibody, NHL repeat containing 1 antibody, NHL repeat containing E3 ubiquitin protein ligase 1 antibody, NHL repeat containing protein 1 antibody, NHL repeat-containing protein 1 antibody, NHL repeat-containing protein 1 gene antibody, NHLC1_HUMAN antibody, NHLRC 1 antibody, Nhlrc1 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q6VVB1
• Puffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: NHLRC1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:1:20-1:200,IF:1:1:20-1:200

Bilder

Immunofluorescence staining of MCF7 cell with CSB-PA740936ESR2HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA740936ESR2HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-PA740936ESR2HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: Rat Liver tissue lysate(20µg), HepG2 whole cell lysate(20µg)
All lanes: NHLRC1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 300s