VPS39 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: VPS39 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA836235LA01HU
• Hersteller Artikelnummer: CSB-PA836235LA01HU
• Alternativnummer: CSB-PA836235LA01HU-100UG, CSB-PA836235LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: A230065P22Rik antibody, AW743070 antibody, FLJ21681 antibody, FLJ46546 antibody, hVam6p antibody, KIAA0770 antibody, RP23-348L16.12 antibody, TLP antibody, TRAP1 like protein antibody, Vacuolar protein sorting 39 antibody, Vacuolar protein sorting 39 homolog antibody, VAM6 antibody, Vam6/Vps39 like antibody, Vam6/Vps39 like protein antibody, Vam6/Vps39-like protein antibody, VPS 39 antibody, Vps39 antibody, VPS39_HUMAN antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q96JC1
• Puffer: Preservative: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: VPS39

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:3000, IHC:1:200-1:500, IF:1:20-1:100

Bilder

Immunofluorescence staining of A549 cell with CSB-PA836235LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with CSB-PA836235LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA836235LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), U251 whole cell lysate(20µg), 293 whole cell lysate(20µg)
All lanes: VPS39 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time:120s