ZDHHC1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ZDHHC1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA837848LA01HU
• Hersteller Artikelnummer: CSB-PA837848LA01HU
• Alternativnummer: CSB-PA837848LA01HU-100UG, CSB-PA837848LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: C16orf1 antibody, DHHC domain containing cysteine rich protein antibody, DHHC domain-containing cysteine-rich protein 1 antibody, DHHC-1 antibody, HSU90653 antibody, Probable palmitoyltransferase ZDHHC1 antibody, ZDHC1_HUMAN antibody, Zdhhc1 antibody, Zinc finger DHHC domain containing 1 antibody, Zinc finger DHHC domain-containing protein 1 antibody, Zinc finger DHHC type containing 1 antibody, Zinc finger protein 377 antibody, ZNF377 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q8WTX9
• Puffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: ZDHHC1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:3000, IHC:1:50-1:200, IF:1:20-1:100

Bilder

Immunofluorescence staining of U251 cell with CSB-PA837848LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with CSB-PA837848LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ImmunofluorescencestainingofA549cellwith5%goatserum,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehyde,permeabilizedusing0.2%TritonX-100andblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).

IHC image of CSB-PA837848LA01HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), U251 whole cell lysate(20µg), 293 whole cell lysate(20µg), Mouse Brain whole cell lysate(20µg),Mouse Spleen tissue lysate(20µg)
All lanes: ZDHHC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 55 kDa
Observed band size: 55 kDa
Exposure time: 10s