CLCC1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CLCC1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA857024LA01HU
• Hersteller Artikelnummer: CSB-PA857024LA01HU
• Alternativnummer: CSB-PA857024LA01HU-100UG, CSB-PA857024LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: CLCC1 antibody, KIAA0761 antibody, MCLCChloride channel CLIC-like protein 1 antibody, Mid-1-related chloride channel protein 1 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q96S66
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: CLCC1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescence staining of Hela cells with CSB-PA857024LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA857024LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA857024LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Colo320 whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate
All lanes: CLCC1 antibody at 3.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63, 57, 48, 40 kDa
Observed band size: 63 kDa

Immunoprecipitating CLCC1 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA857024LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA857024LA01HU (6µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)