HIF1AN Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HIF1AN Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA865160LA01HU
• Hersteller Artikelnummer: CSB-PA865160LA01HU
• Alternativnummer: CSB-PA865160LA01HU-100UG, CSB-PA865160LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: DKFZp762F1811 antibody, Factor inhibiting HIF-1 antibody, Factor inhibiting HIF1 antibody, FIH 1 antibody, FIH-1 antibody, FIH1 antibody, FLJ20615 antibody, FLJ22027 antibody, HIF1AN antibody, HIF1N_HUMAN antibody, Hypoxia inducible factor 1 alpha inhibitor antibody, Hypoxia inducible factor 1 alpha subunit inhibitor antibody, Hypoxia inducible factor asparagine hydroxylase antibody, Hypoxia-inducible factor 1-alpha inhibitor antibody, Hypoxia-inducible factor asparagine hydroxylase antibody, Peptide aspartate beta dioxygenase antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9NWT6
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: HIF1AN

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescent analysis of Hela cells using CSB-PA865160LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA865160LA01HU at dilution of 1:100

Immunoprecipitating HIF1AN in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA865160LA01HU in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA865160LA01HU (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (10µg)

Western blot
All lanes: HIF1AN antibody at 12µg/ml
Lane 1: Mouse heart tissue
Lane 2: Mouse skeletal muscle tissue
Lane 3: K562 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: HL60 whole cell lysate
Lane 6: 293T whole cell lysate
Lane 7: Jurkat whole cell lysate
Lane 8: A375 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41, 48 kDa

Immunofluorescent analysis of MCF-7 cells using CSB-PA865160LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)