TUBGCP4 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TUBGCP4 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA871402LA01HU
• Hersteller Artikelnummer: CSB-PA871402LA01HU
• Alternativnummer: CSB-PA871402LA01HU-100UG, CSB-PA871402LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: TUBGCP4 antibody, 76P antibody, GCP4 antibody, Gamma-tubulin complex component 4 antibody, GCP-4 antibody, hGCP4 antibody, Gamma-ring complex protein 76 kDa antibody, h76p antibody, hGrip76 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9UGJ1
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: TUBGCP4

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:200-1:500, IP:1:200-1:2000

Bilder

Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA871402LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871402LA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA871402LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating TUBGCP4 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA871402LA01HU in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA871402LA01HU (8ug) + MCF-7 whole cell lysate (500ug)
Lane 3: MCF-7 whole cell lysate (10ug)

Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: TUBGCP4 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa