PACSIN2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PACSIN2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA890765EA01HU
• Hersteller Artikelnummer: CSB-PA890765EA01HU
• Alternativnummer: CSB-PA890765EA01HU-100UG, CSB-PA890765EA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: cytoplasmic phosphoprotein PACSIN2 antibody, OTTHUMP00000198281 antibody, OTTHUMP00000198282 antibody, OTTHUMP00000198283 antibody, OTTHUMP00000198284 antibody, PACN2_HUMAN antibody, Pacsin2 antibody, protein kinase C and casein kinase substrate in neurons 2 antibody, Protein kinase C and casein kinase substrate in neurons protein 2 antibody, SDPII antibody, Syndapin II antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9UNF0
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: PACSIN2

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescence staining of Hela cells with CSB-PA890765EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA890765EA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA890765EA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating PACSIN2 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA890765EA01HU in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA890765EA01HU (6ug) + HepG2 whole cell lysate (500ug)
Lane 3: HepG2 whole cell lysate (20ug)

Western Blot
Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate
All lanes: PACSIN2 antibody at 2.6ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56, 52 kDa
Observed band size: 56 kDa