VPS4A Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: VPS4A Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA891974DSR2HU
• Hersteller Artikelnummer: CSB-PA891974DSR2HU
• Alternativnummer: CSB-PA891974DSR2HU-100UL, CSB-PA891974DSR2HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: hVPS4 antibody, Protein SKD2 antibody, SKD1 antibody, SKD1 homolog antibody, SKD1A antibody, SKD2 antibody, Vacuolar protein sorting 4 homolog A antibody, Vacuolar protein sorting 4, yeast, homolog of, A antibody, Vacuolar protein sorting factor 4A antibody, Vacuolar protein sorting-associated protein 4A antibody, vacuolar sorting protein 4 antibody, VPS4 antibody, VPS4-1 antibody, Vps4a antibody, VPS4A_HUMAN antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9UN37
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: VPS4A

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Bilder

IHC image of CSB-PA891974DSR2HU diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA891974DSR2HU diluted at 1:150 and staining in paraffin-embedded human prostatecancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: U87 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), A549 whole cell lysate(20µg), 293 whole cell lysate(20µg)
All lanes: VPS4A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 120s