MAGED2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MAGED2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA891976LA01HU
• Hersteller Artikelnummer: CSB-PA891976LA01HU
• Alternativnummer: CSB-PA891976LA01HU-100UG, CSB-PA891976LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: 11B6 antibody, BCG 1 antibody, BCG-1 antibody, Breast cancer-associated gene 1 protein antibody, HCA10 antibody, Hepatocellular carcinoma-associated protein JCL-1 antibody, JCL 1 antibody, MAGD2_HUMAN antibody, MAGE D antibody, MAGE D2 antigen antibody, MAGE-D2 antigen antibody, MAGED2 antibody, Melanoma antigen family D 2 antibody, Melanoma associated antigen D2 antibody, Melanoma-associated antigen D2 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q9UNF1
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: MAGED2

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescence staining of Hela cells with CSB-PA891976LA01HU at 1:145, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA891976LA01HU diluted at 1:320 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating MAGED2 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA891976LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA891976LA01HU (6µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate
All lanes: MAGED2 antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65, 64 kDa
Observed band size: 65, 64 kDa