Phospho-AKT1 (Ser473) Recombinant Monoclonal Antibody, Clone: [4H12], Unconjugated, Rabbit
Artikelnummer:
CSB-RA001553A473PHHU
- Bilder (5)
| Artikelname: | Phospho-AKT1 (Ser473) Recombinant Monoclonal Antibody, Clone: [4H12], Unconjugated, Rabbit |
| Artikelnummer: | CSB-RA001553A473PHHU |
| Hersteller Artikelnummer: | CSB-RA001553A473phHU |
| Alternativnummer: | CSB-RA001553A473PHHU-100UL, CSB-RA001553A473PHHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [4H12] |
| UniProt: | P31749 |
| Puffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Reinheit: | Affinity-chromatography |
| Formulierung: | Liquid |
| Target-Kategorie: | AKT1 |
| Antibody Type: | Recombinant Antibody |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000 |
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Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with CSB-RA001553A473phHU at 1:68,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) . |
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IHC image of CSB-RA001553A473phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-RA001553A473phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating Phospho-AKT1 in 293 whole cell lysate treated with Calyculin A Lane 1: Rabbit control IgG(1µg) instead of CSB-RA001553A473phHU in 293 whole cell lysate treated with Calyculin A.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA001553A473phHU(3µg) + 293 whole cell lysate treated with Calyculin A(1mg) Lane 3: 293 whole cell lysate treated with Calyculin A (20µg) |
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Western Blot Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not) All lanes Phospho-AKT1 antibody at 1.08µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60 KDa Observed band size: 60 KDa |
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