CLU Recombinant Monoclonal Antibody, Clone: [19D11], Unconjugated

Produktübersicht

• Artikelname: CLU Recombinant Monoclonal Antibody, Clone: [19D11], Unconjugated
• Artikelnummer: CSB-RA005595MA2HU
• Hersteller Artikelnummer: CSB-RA005595MA2HU
• Alternativnummer: CSB-RA005595MA2HU-100UL, CSB-RA005595MA2HU-50UL
• Hersteller: Cusabio
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Clusterin (Aging-associated gene 4 protein) (Apolipoprotein J) (Apo-J) (Complement cytolysis inhibitor) (CLI) (Complement-associated protein SP-40,40) (Ku70-binding protein 1) (NA1/NA2) (Testosterone-repressed prostate message 2) (TRPM-2) [Cleaved into: Clusterin beta chain (ApoJalpha) (Complement cytolysis inhibitor a chain) , Clusterin alpha chain (ApoJbeta) (Complement cytolysis inhibitor b chain) ], CLU, APOJ CLI KUB1

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [19D11]
• UniProt: P10909
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: CLU
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing HepG2 cells stained with CSB-RA005595MA2HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific at 1:200 dilution for 35 min at 4°C.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of A549 cell with CSB-RA005595MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .

Immunofluorescence staining of Hela cell with CSB-RA005595MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .

IHC image of CSB-RA005595MA2HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA005595MA2HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA005595MA2HU diluted at 1:50 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: HeLa whole cell lysate(30µg) , HepG2 whole cell lysate(30µg) , colo205 whole cell lysate(30µg)
All lanes: Clusterin antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 30s