GDNF Recombinant Monoclonal Antibody, Clone: [11D2], Unconjugated, Rabbit
Artikelnummer:
CSB-RA009356A0HU
- Bilder (5)
| Artikelname: | GDNF Recombinant Monoclonal Antibody, Clone: [11D2], Unconjugated, Rabbit |
| Artikelnummer: | CSB-RA009356A0HU |
| Hersteller Artikelnummer: | CSB-RA009356A0HU |
| Alternativnummer: | CSB-RA009356A0HU-100UL, CSB-RA009356A0HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IHC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [11D2] |
| UniProt: | P39905 |
| Puffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Reinheit: | Affinity-chromatography |
| Formulierung: | Liquid |
| Target-Kategorie: | GDNF |
| Antibody Type: | Recombinant Antibody |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
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Overlay histogram showing SH-SY5Y cells stained with CSB-RA009356A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
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IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating GDNF in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA009356A0HU in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA009356A0HU (3µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg) |
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Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate All lanes: GDNF antibody at 1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24, 21, 26, 23, 19 KDa Observed band size: 24 KDa |
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