PRDX3 Recombinant Monoclonal Antibody, Clone: [7G2], Unconjugated

Produktübersicht

• Artikelname: PRDX3 Recombinant Monoclonal Antibody, Clone: [7G2], Unconjugated
• Artikelnummer: CSB-RA018656MA1HU
• Hersteller Artikelnummer: CSB-RA018656MA1HU
• Alternativnummer: CSB-RA018656MA1HU-100UL, CSB-RA018656MA1HU-50UL
• Hersteller: Cusabio
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Antioxidant protein 1 antibody, AOP 1 antibody, AOP-1 antibody, AOP1 antibody, HBC189 antibody, MER5 antibody, MGC104387 antibody, MGC24293 antibody, mitochondrial antibody, peroxiredoxin 3 antibody, Peroxiredoxin III antibody, Peroxiredoxin-3 antibody, PRDX3 antibody, PRDX3_HUMAN antibody, PRO1748 antibody, Protein MER5 homolog antibody, PRX III antibody, Prx-III antibody, PRX3 antibody, SP 22 antibody, SP-22 antibody, SP22 antibody, Thioredoxin dependent peroxide reductase mitochondrial antibody, Thioredoxin-dependent peroxide reductase antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [7G2]
• UniProt: P30048
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: PRDX3
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing HepG2 cells stained with CSB-RA018656MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific at 1:200 dilution for 35 min at 4°C.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cell with CSB-RA018656MA1HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific.

IHC image of CSB-RA018656MA1HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA018656MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA018656MA1HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: HEK293 whole cell lysate(30µg), colo205 whole cell lysate(30µg), A-431 whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg)
All lanes: PRDX3 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 28 kDa
Observed band size: 25 kDa
Exposure time: 20s