TUBB Recombinant Monoclonal Antibody, Clone: [16E11D4], Unconjugated

Produktübersicht

• Artikelname: TUBB Recombinant Monoclonal Antibody, Clone: [16E11D4], Unconjugated
• Artikelnummer: CSB-RA025318MA1HU
• Hersteller Artikelnummer: CSB-RA025318MA1HU
• Alternativnummer: CSB-RA025318MA1HU-100UL, CSB-RA025318MA1HU-50UL
• Hersteller: Cusabio
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: Beta 4 tubulin antibody, Beta 5 tubulin antibody, beta Ib tubulin antibody, Beta1 tubulin antibody, Class I beta tubulin antibody, M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW cl.56 antibody, OK/SWcl.56 antibody, TBB5_HUMAN antibody, TUBB 1 antibody, TUBB 2 antibody, TUBB 5 antibody, TUBB antibody, TUBB1 antibody, TUBB2 antibody, TUBB5 antibody, tubulin beta 1 chain antibody, Tubulin beta 2 chain antibody, tubulin beta 5 chain antibody, Tubulin beta chain antibody, Tubulin beta class I antibody, tubulin beta polypeptide antibody, Tubulin beta-5 chain antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [16E11D4]
• UniProt: P07437
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: TUBB
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing K562 cells stained with CSB-RA025318MA1HU (red line) at 1:100.The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ImmunofluorescencestainingofHelacellwithCSB-RA025318MA1HUat1:100,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasFITC-conjugatedAffiniPureGoatAnti-HumanIgG(H+L) .

IHC image of CSB-RA008615MA1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA008615MA1HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA008615MA1HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western BlotPositive WB detected in:A431 whole cell lysate (20µg) , SH-SY5Y whole cell lysate (20µg) , U251 whole cell lysate (20µg) , 293T whole cell lysate (20µg) , HepG2 whole cell lysate (20µg) , K562 whole cell lysate (20µg) , MCF7 whole cell lysate (20µg) , NIH/3T3 whole cell lysate (20µg) All lanes: TUBB antibody at 1:1000SecondaryGoat polyclonal to human IgG at 1/40000 dilutionPredicted band size:49 kDaObserved band size:55 kDaExposure time:5s