NOLC1 Recombinant Monoclonal Antibody, Clone: [1D9], Unconjugated, Rabbit

Produktübersicht

• Artikelname: NOLC1 Recombinant Monoclonal Antibody, Clone: [1D9], Unconjugated, Rabbit
• Artikelnummer: CSB-RA027798A0HU
• Hersteller Artikelnummer: CSB-RA027798A0HU
• Alternativnummer: CSB-RA027798A0HU-100UL, CSB-RA027798A0HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: 140 kDa nucleolar phosphoprotein antibody, HCV NS5A trans regulated protein 13 antibody, HCV NS5A transactivated protein 13 antibody, HCV NS5A-transactivated protein 13 antibody, Hepatitis C virus NS5A transactivated protein 13 antibody, Hepatitis C virus NS5A-transactivated protein 13 antibody, KIAA0035 antibody, NOLC 1 antibody, NOLC1 antibody, NOLC1_HUMAN antibody, NOPP 130 antibody, NOPP 140 antibody, NOPP130 antibody, Nopp140 antibody, NS5ATP13 antibody, Nucleolar 130 kDa protein antibody, Nucleolar and coiled body phosphoprotein 1 antibody, Nucleolar and coiled body phosphprotein 1 antibody, Nucleolar and coiled-body phosphoprotein 1 antibody, Nucleolar phosphoprotein p130 antibody, Nucleolar protein p130 antibody, P130 antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [1D9]
• UniProt: Q14978
• Puffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: NOLC1
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing HepG2 cells stained with CSB-RA027798A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunofluorescence staining of Hela cell with CSB-RA027798A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-RA027798A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg)
All lanes: NOLC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 74 kDa
Observed band size: 110 kDa
Exposure time:1min