ZBTB7A Recombinant Monoclonal Antibody, Clone: [8D5], Unconjugated, Rabbit

Produktübersicht

• Artikelname: ZBTB7A Recombinant Monoclonal Antibody, Clone: [8D5], Unconjugated, Rabbit
• Artikelnummer: CSB-RA063930A0HU
• Hersteller Artikelnummer: CSB-RA063930A0HU
• Alternativnummer: CSB-RA063930A0HU-100UL, CSB-RA063930A0HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Zinc finger and BTB domain-containing protein 7A (Factor binding IST protein 1) (FBI-1) (Factor that binds to inducer of short transcripts protein 1) (HIV-1 1st-binding protein 1) (Leukemia/lymphoma-related factor) (POZ and Krueppel erythroid myeloid ontogenic factor) (POK erythroid myeloid ontogenic factor) (Pokemon) (TTF-I-interacting peptide 21) (TIP21) (Zinc finger protein 857A) , ZBTB7A, FBI1 LRF ZBTB7 ZNF857A

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [8D5]
• UniProt: O95365
• Puffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: ZBTB7A
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing BxPC-3 cells stained with CSB-RA063930A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of MCF-7 cell with CSB-RA063930A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: A431 whole cell lysate(30µg) , MCF-7 whole cell lysate(30µg) , HT-29 whole cell lysate(30µg) , THP-1 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg) , HeLa whole cell lysate(30µg) , A549 whole cell lysate(30µg)
All lanes: ZBTB7A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 61 kDa
Observed band size: 70 kDa
Exposure time:2min