N Recombinant Monoclonal Antibody, Clone: [1A6], Unconjugated, Mouse

Produktübersicht

• Artikelname: N Recombinant Monoclonal Antibody, Clone: [1A6], Unconjugated, Mouse
• Artikelnummer: CSB-RA33255A1GMY
• Hersteller Artikelnummer: CSB-RA33255A1GMY
• Alternativnummer: CSB-RA33255A1GMY-100UL, CSB-RA33255A1GMY-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, GICA, WB
• Spezies Reaktivität: Virus
• Konjugation: Unconjugated
• Alternative Synonym: Nucleocapsid protein, NC, protein N, N

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [1A6]
• UniProt: P0DTC9
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: N
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: ELISA:1:10000-1:50000, WB:1:500-1:5000, GICA:1:1000-1:10000

Bilder

In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml) , and the sensitivity is very good.

ELISA: Immobilize various types of SARS proteins at concentration of 2µg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100µg/ml, 10µg/ml and 1µg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.

The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 µg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 µg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 µg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 µg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.

Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa