CDK6 Recombinant Monoclonal Antibody, Clone: [8G3], Unconjugated, Rabbit

Produktübersicht

• Artikelname: CDK6 Recombinant Monoclonal Antibody, Clone: [8G3], Unconjugated, Rabbit
• Artikelnummer: CSB-RA555745A0HU
• Hersteller Artikelnummer: CSB-RA555745A0HU
• Alternativnummer: CSB-RA555745A0HU-100UL, CSB-RA555745A0HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE) , CDK6, CDKN6

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [8G3]
• UniProt: Q00534
• Puffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Reinheit: Affinity-chromatography
• Formulierung: Liquid
• Target-Kategorie: CDK6
• Antibody Type: Recombinant Antibody

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:100, IF:1:10-1:100, IP:1:1000-1:2000

Bilder

Immunofluorescence staining of U251 cell with CSB-RA555745A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of Hela cell with CSB-RA555745A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

IHC image of CSB-RA555745A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA555745A0HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Immunoprecipitating CDK6 in K562whole cell lysate
Lane 1: CSB-RA555745A0HU(3µg) + K562 whole cell lysate(220µg)
Lane 2: K562 whole cell lysate(30µg)
Lane 3:Rabbit control IgG instead of CSB-RA010605A0HUinK562wholecelllysate
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/40000)

Western Blot
Positive WB detected in: JK whole cell lysate(20µg) , K562 whole cell lysate(20µg) , SY5Y whole cell lysate(20µg) , HepG2 whole cell lysate(20µg) , COLO205 whole cell lysate(20µg)
All lanes: CDK6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 120s