Anti-Guinea Pig IgG F(ab)2 Peroxidase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab)2 molecules lack the
Klonalität:
Polyclonal
Konzentration:
10.0 mg/mL
Isotyp:
Ig
Puffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab’)2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c).
Formulierung:
Lyophilized
Formel:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target-Kategorie:
Guinea Pig
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:10,000 - 1:50,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:1,000 - 1:10,000
Anwendungsbeschreibung:
This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
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