F(ab)2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimen
Klonalität:
Polyclonal
Konzentration:
10.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
Formulierung:
Lyophilized
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target-Kategorie:
Chicken
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:40,000 - 1:160,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung:
F(ab)2 Chicken IgG Antibody Peroxidase Conjugated is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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