F(ab)2 Anti-Guinea Pig IgG Peroxidase Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunoch
Klonalität:
Polyclonal
Konzentration:
10.0 mg/mL
Isotyp:
Ig
Puffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
Formulierung:
Lyophilized
Formel:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,Azide/BSA free
Target-Kategorie:
Guinea Pig
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:10,000 - 1:50,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:1,000 - 1:10,000
Anwendungsbeschreibung:
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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