goat anti-Dog IgG F(c) Antibody Biotin conjugation, goat anti-Dog IgG Fc fragment biotin conjugated Antibody, Dog Secondary Antibody
Format:
IgG
Spezifität:
IgG (Fc)
Minimale Kreuzreaktivität (MinX):
no cross-adsorbtion
Anti-DOG IgG F(c) (GOAT) Antibody generated in Goats detects specifically Dog IgG F(c). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bact
Klonalität:
Polyclonal
Konzentration:
2.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
Anti-DOG IgG F(c) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Dog IgG, Dog IgG F(c) and Dog Serum. No reaction was observed against Dog IgG F(ab).
Formulierung:
Lyophilized
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target-Kategorie:
Dog
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung:
Anti-DOG IgG F(c) (GOAT) Antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 is suggested for this product.
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