Goat Anti-Guinea Pig IgG F(ab)2 Biotin Conjugated Antibody, Goat Anti Guinea Pig IgG F(ab)2 Antibody Biotin Conjugated , Goat Anti Guinea Pig IgG Fab2 Fragment Biotin Conjugated Antibody
Format:
IgG
Spezifität:
IgG (F(ab')2)
Minimale Kreuzreaktivität (MinX):
no cross-adsorbtion
Anti-Guinea Pig IgG F(ab)2 Biotin Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab)2 molecules lack the Fc po
Klonalität:
Polyclonal
Konzentration:
2.0 mg/mL
Isotyp:
Ig
Puffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(ab’)2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c).
Formulierung:
Lyophilized
Formel:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target-Kategorie:
Guinea Pig
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung:
This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:28,000 to 1:114,000 of the reconstitution concentration is suggested for this product.
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