Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.
Konjugation:
Alk. Phos.
Alternative Synonym:
Human Fc Antibody, Rb-a-Human F(c), Human IgG F(c) Antibody in Rabbit, rabbit anti-Human IgG F(c) Alkaline phosphatase Secondary Antibody
Format:
IgG
Spezifität:
IgG (Fc)
Minimale Kreuzreaktivität (MinX):
no cross-adsorbtion
Anti-Human IgG F(c) Alkaline Phosphatase Conjugated generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Rec
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Isotyp:
Ig
Puffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Reinheit:
Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
Formulierung:
Liquid (sterile filtered)
Formel:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0.05% NaN3
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:2,000 - 1:10,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:500 - 1:2,500
Anwendungsbeschreibung:
Anti-Human IgG F(c) antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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