Anti-Human IgG (H&L) Peroxidase generated in donkey detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two ident
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
Anti-HUMAN IgG (H&L) (DONKEY) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Formulierung:
Lyophilized
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:10,000 - 1:100,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:1,000 - 1:5,000
Anwendungsbeschreibung:
Anti-HUMAN IgG (H&L) (DONKEY) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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