Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to
Klonalität:
Polyclonal
Konzentration:
1.0 mg/ml
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Formulierung:
Liquid (sterile filtered)
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target-Kategorie:
Mouse
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung:
Anti-Mouse IgG subclass pan reactive Secondary Antibody is designed for ELISA, Immunohistochemistry, western blotting as well as other anti-IgG antibody based assays. Mouse IgG secondary antibody is available in a variety of formats.
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