Anti-Rabbit IgG F(c) fluorescein antibody generated in donkey is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion
Klonalität:
Polyclonal
Konzentration:
2.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
Formulierung:
Lyophilized
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target-Kategorie:
Rabbit
Antibody Type:
Secondary Antibody
Application Verdünnung:
FLISA Dilution: 1:100,000, Fluorochrome Protein Value: 4.07, IF Microscopy Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:20,000
Anwendungsbeschreibung:
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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