Chicken IgG anti-Rat IgG (H+L)-Biotin, MinX none

Artikelnummer: DNA-SEC-183535
Artikelname: Chicken IgG anti-Rat IgG (H+L)-Biotin, MinX none
Artikelnummer: DNA-SEC-183535
Hersteller Artikelnummer: SEC-183535
Alternativnummer: DNA-SEC-183535
Hersteller: dianova
Wirt: Gallus
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Rat
Immunogen: Rat IgG whole molecule
Konjugation: Biotin
Alternative Synonym: Chicken Anti Rat IgG Biotin Conjugated Antibody, Chicken Anti-Rat IgG Antibody Biotin Conjugation
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Anti-Rat IgG (H&L) generated in chicken detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the ci
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL
Isotyp: Ig
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken Serum, Rat IgG and Rat Serum.
Formulierung: Lyophilized
Formel: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target-Kategorie: Rat
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.