Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.
Konjugation:
Unconjugated
Alternative Synonym:
Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
Format:
IgG
Spezifität:
IgG (Fc)
Minimale Kreuzreaktivität (MinX):
no cross-adsorbtion
Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of sheep IgG and often
Klonalität:
Polyclonal
Konzentration:
2.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab).
Formulierung:
Liquid (sterile filtered)
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target-Kategorie:
Sheep
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:10,000 - 1:200,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:1,000 - 1:5,000
Anwendungsbeschreibung:
Anti-Sheep IgG F(c) antibody has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
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