F(ab)2 Anti-Goat IgG Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Isotyp:
Ig
Puffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Reinheit:
F(ab')2 Anti-GOAT IgG (H&L) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. F(ab')2 Anti-GOAT IgG (H&L) (RABBIT) Antibody assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Formulierung:
Liquid (sterile filtered)
Formel:
10 mM NaPO4,250 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target-Kategorie:
Goat
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung:
F(ab')2 Anti-GOAT IgG (H&L) (RABBIT) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Specific conditions should be optimized by researcher.
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