F(ab)2 Anti-Human IgG (H&L) Antibody generated in goat detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
F(ab)2 Anti-Human IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Mouse and Rat Serum Proteins.
Formulierung:
Liquid (sterile filtered)
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:42,000, Immunohistochemistry Dilution: 1:500 - 1:2,000, Western Blot Dilution: 1:1,000 - 1:5,000
Anwendungsbeschreibung:
F(ab)2 Anti-Human IgG (H&L) Antibody has been tested by ELISA and dot blot and is suitable for ELISA, immunoblotting, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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