F(ab)2 Anti-Rabbit IgG (H&L) Antibody generated in goat detects rabbit IgG. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
Formulierung:
Lyophilized
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target-Kategorie:
Rabbit
Antibody Type:
Secondary Antibody
Application Verdünnung:
FLISA Dilution: 1:10,000 - 1:50,000, Flow Cytometry Dilution: 1:500-1:2,500, Fluorochrome Protein Value: 4.9, IF Microscopy Dilution: 1:1,000 - 1:5,000
Anwendungsbeschreibung:
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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