Human AGE(Advanced Glycation End Product) ELISA Kit
Artikelnummer:
EBT-ELK0081
Hersteller Artikelnummer:
ELK0081
Alternativnummer:
EBT-ELK0081-96, EBT-ELK0081-48, EBT-ELK0081-96X5
Hersteller:
ELK Biotechnology
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
AGEs, Advanced Glycation End Products
Konzentration:
8000 ng/mL
Detektionsbereich:
125-8000 ng/mL
Sensitivitaet:
38.2 ng/mL
Proben:
serum, plasma and other biological fluids
Anwendungsbeschreibung:
Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Metabolic pathway,Endocrinology,Cardiovascular biology,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human AGE in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0081
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