Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit

Produktübersicht

• Artikelname: Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
• Artikelnummer: EBT-ELK0117
• Hersteller Artikelnummer: ELK0117
• Alternativnummer: EBT-ELK0117-96, EBT-ELK0117-48, EBT-ELK0117-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: ADD-1, ADD1, SREBP-1, SREBP-1c, Srebp1

Produkteigenschaften

• Konzentration: 4000 pg/mL
• Detektionsbereich: 62.5-4000 pg/mL
• Sensitivitaet: 18.3 pg/mL
• Proben: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular, Epigenetics and Nuclear Signaling, Cancer, Metabolism. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SREBP-1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SREBP-1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SREBP-1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat SREBP-1C in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK0117