EasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Artikelnummer: EBT-ELK035ES
Artikelname: EasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Artikelnummer: EBT-ELK035ES
Hersteller Artikelnummer: ELK035ES
Alternativnummer: EBT-ELK035ES-96, EBT-ELK035ES-48, EBT-ELK035ES-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: NT-Pro-BNP, N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb
Konzentration: 60 ng/mL
Detektionsbereich: 0.94-60 ng/mL
Sensitivitaet: 0.94 ng/mL
Proben: Serum, plasma
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 1.5h. Research Area: Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve